Hotspot

petN-psbM

LSC · 29182-30447 · 1266 bp

Back to Dimocarpus

Pi 0.0079

MarkerSeek score 60.60

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1266
variable_sites: 10
pi: 0.0079
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1428-1448 bp	1.0	68.1
petN-psbM_p2	TATGGGGAAGGAGTGGACTC	TGCTACTGCACTGTTCATTCT	55.0	42.9	57.8	57.6	1368-1388 bp	1.0	67.9
petN-psbM_p3	GGGGAAGGAGTGGACTCTAG	TGCTACTGCACTGTTCATTCT	60.0	42.9	58.2	57.6	1365-1385 bp	1.0	67.6
petN-psbM_p4	CTTGGGCTGCGTTAATGGTA	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.3	57.6	1416-1436 bp	1.0	67.3
petN-psbM_p5	TTGGGCTGCGTTAATGGTAG	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.3	57.6	1415-1435 bp	1.0	67.3