Hotspot

atpH-atpI

LSC · 13791-14993 · 1203 bp

Back to Dimocarpus

Pi 0.00333

MarkerSeek score 60.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1203
variable_sites: 4
pi: 0.00333
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AATAGAAGCAAGCCCGACAG	AGTGGTTCCGATACCTGTCA	50.0	50.0	58.0	58.1	1384-1385 bp	1.0	79.5
atpH-atpI_p2	AATAGAAGCAAGCCCGACAG	AAGCGGCATTCAAGCTCTTA	50.0	45.0	58.0	57.9	1345-1346 bp	1.0	79.1
atpH-atpI_p3	AATAGAAGCAAGCCCGACAG	TTTTGCAACTTTAGCTGCGG	50.0	45.0	58.0	57.9	1324-1325 bp	1.0	79.0
atpH-atpI_p4	TACCTTGACCAACTCCAGGT	AGTGGTTCCGATACCTGTCA	50.0	50.0	57.9	58.1	1406-1407 bp	1.0	78.9
atpH-atpI_p5	TACCTTGACCAACTCCAGGT	AAGCGGCATTCAAGCTCTTA	50.0	45.0	57.9	57.9	1367-1368 bp	1.0	78.5