Hotspot

rpl16

LSC · 85167-86655 · 1489 bp

Back to Dillenia

Pi 0.00417

MarkerSeek score 64.40

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1489
variable_sites: 6
pi: 0.00417
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	AAAAAGGAACCTGCCGTTGT	TCTGTTCAATTAAAACCCAAATGAGT	45.0	30.8	58.5	58.0	1657-1813 bp	1.0	42.6
rpl16_p2	AAAAGGAACCTGCCGTTGTT	TCTGTTCAATTAAAACCCAAATGAGT	45.0	30.8	58.5	58.0	1656-1812 bp	1.0	42.6
rpl16_p3	AAAGGAACCTGCCGTTGTTT	TCTGTTCAATTAAAACCCAAATGAGT	45.0	30.8	58.5	58.0	1655-1811 bp	1.0	42.6
rpl16_p4	AAGGAACCTGCCGTTGTTTT	TCTGTTCAATTAAAACCCAAATGAGT	45.0	30.8	58.5	58.0	1654-1810 bp	1.0	42.6
rpl16_p5	AGGAACCTGCCGTTGTTTTT	TCTGTTCAATTAAAACCCAAATGAGT	45.0	30.8	58.5	58.0	1653-1809 bp	1.0	42.6