Hotspot

trnL-CAA-ycf15

IRa · 149119-149746 · 628 bp

Back to Dillenia

Pi 0.00955
MarkerSeek score 64.20
Primer available yes
Label trnL-CAA-ycf15

Feature Metadata

label_name
trnL-CAA-ycf15
feature_type
igs
region
IRa
length_bp
628
variable_sites
6
pi
0.00955
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-CAA-ycf15_p1 AAATGGTAGACACGCGAGAC ACGCATGTCTGATGAAATGGT 50.0 42.9 58.0 58.3 759 bp 1.0 70.3
trnL-CAA-ycf15_p2 TGAAATGGTAGACACGCGAG ACGCATGTCTGATGAAATGGT 50.0 42.9 58.0 58.3 761 bp 1.0 70.3
trnL-CAA-ycf15_p3 GCCTTGGTGGTGAAATGGTA ACGCATGTCTGATGAAATGGT 50.0 42.9 58.1 58.3 771 bp 1.0 69.7
trnL-CAA-ycf15_p4 AAATGGTAGACACGCGAGAC GGTTGTTGCTATTTGCTCCA 50.0 45.0 58.0 56.6 741 bp 1.0 69.4
trnL-CAA-ycf15_p5 AAATGGTAGACACGCGAGAC TGGTTGTTGCTATTTGCTCC 50.0 45.0 58.0 56.6 742 bp 1.0 69.4