Hotspot

rpl32-trnL-UAG

SSC · 117522-118493 · 972 bp

Back to Dillenia

Pi 0.0669

MarkerSeek score 75.60

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 972
variable_sites: 65
pi: 0.0669
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AAGGGTTGTTGGTCAGCATT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.9	58.0	1094-1592 bp	1.0	83.2
rpl32-trnL-UAG_p2	AAGGGTTGTTGGTCAGCATT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.9	58.0	1095-1593 bp	1.0	83.2
rpl32-trnL-UAG_p3	AAAAGGGACAATCCACGCAT	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.1	58.0	1544-2928 bp	1.0	82.1
rpl32-trnL-UAG_p4	AAAAGGGACAATCCACGCAT	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.1	58.0	1545-2929 bp	1.0	82.1
rpl32-trnL-UAG_p5	GGACAATCCACGCATATCGA	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.9	58.0	1539-2923 bp	1.0	81.5