Hotspot

trnR-ACG-trnN-GUU

IRb · 112622-113227 · 606 bp

Back to Dillenia

Pi 0.00864

MarkerSeek score 68.00

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 606
variable_sites: 5
pi: 0.00864
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	720-747 bp	1.0	81.7
trnR-ACG-trnN-GUU_p2	CAGAGGATTAGAGCACGTGG	TGGGTTGAGAACGGGAATTG	55.0	50.0	57.8	58.1	761-788 bp	1.0	80.8
trnR-ACG-trnN-GUU_p3	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	671-698 bp	1.0	80.8
trnR-ACG-trnN-GUU_p4	CAGAGGATTAGAGCACGTGG	GGGTTGAGAACGGGAATTGA	55.0	50.0	57.8	57.8	760-787 bp	1.0	80.0
trnR-ACG-trnN-GUU_p5	CAGAGGATTAGAGCACGTGG	CGGTCGGCTGTTAACTGATT	55.0	50.0	57.8	58.3	690-717 bp	1.0	79.7