Hotspot

ycf15-trnL-CAA

IRb · 97826-98453 · 628 bp

Back to Dillenia

Pi 0.00955
MarkerSeek score 64.20
Primer available yes
Label ycf15-trnL-CAA

Feature Metadata

label_name
ycf15-trnL-CAA
feature_type
igs
region
IRb
length_bp
628
variable_sites
6
pi
0.00955
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf15-trnL-CAA_p1 ACGCATGTCTGATGAAATGGT AAATGGTAGACACGCGAGAC 42.9 50.0 58.3 58.0 759 bp 1.0 70.3
ycf15-trnL-CAA_p2 ACGCATGTCTGATGAAATGGT TGAAATGGTAGACACGCGAG 42.9 50.0 58.3 58.0 761 bp 1.0 70.3
ycf15-trnL-CAA_p3 ACGCATGTCTGATGAAATGGT GCCTTGGTGGTGAAATGGTA 42.9 50.0 58.3 58.1 771 bp 1.0 69.7
ycf15-trnL-CAA_p4 GGTTGTTGCTATTTGCTCCA AAATGGTAGACACGCGAGAC 45.0 50.0 56.6 58.0 741 bp 1.0 69.4
ycf15-trnL-CAA_p5 TGGTTGTTGCTATTTGCTCC AAATGGTAGACACGCGAGAC 45.0 50.0 56.6 58.0 742 bp 1.0 69.4