Hotspot

ndhC-trnV-UAC

LSC · 53836-54553 · 718 bp

Back to Dillenia

Pi 0.158
MarkerSeek score 94.40
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
718
variable_sites
113
pi
0.158
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
94.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGGTCCTTTTCTAATCGGAGC GCAGTTTGGCCTGTTTTACC 47.6 50.0 57.7 57.9 886-1103 bp 1.0 77.1
ndhC-trnV-UAC_p2 TGGTCCTTTTCTAATCGGAGC TGGCCTGTTTTACCGAGAAG 47.6 50.0 57.7 57.8 880-1097 bp 1.0 76.9
ndhC-trnV-UAC_p3 TGGTCCTTTTCTAATCGGAGC AGAAGGTCTACGGTTCGAGT 47.6 50.0 57.7 57.8 865-1082 bp 1.0 76.7
ndhC-trnV-UAC_p4 TGGTCCTTTTCTAATCGGAGC AATGAGCAGTTTGGCCTGTT 47.6 45.0 57.7 58.3 891-1108 bp 1.0 76.0
ndhC-trnV-UAC_p5 TGGTCCTTTTCTAATCGGAGC ATGAGCAGTTTGGCCTGTTT 47.6 45.0 57.7 58.3 890-1107 bp 1.0 76.0