Hotspot

trnT-GGU-psbD

LSC · 34142-35387 · 1246 bp

Back to Dillenia

Pi 0.0289

MarkerSeek score 75.40

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1246
variable_sites: 36
pi: 0.0289
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	AATTGAATCAAACGGGCCCT	GAAACGGTCCCTACGTAACC	45.0	55.0	58.1	58.0	1414-1750 bp	1.0	81.3
trnT-GGU-psbD_p2	ATTGAATCAAACGGGCCCTT	GAAACGGTCCCTACGTAACC	45.0	55.0	58.1	58.0	1413-1749 bp	1.0	81.3
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	GAAACGGTCCCTACGTAACC	55.0	55.0	58.1	58.0	1385-1721 bp	1.0	81.3
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	GAAACGGTCCCTACGTAACC	50.0	55.0	58.1	58.0	1388-1724 bp	1.0	81.1
trnT-GGU-psbD_p5	AACGGGCCCTTTTAACTCAG	GAAACGGTCCCTACGTAACC	50.0	55.0	58.1	58.0	1404-1740 bp	1.0	81.0