Hotspot

psbM-trnD-GUC

LSC · 31262-32390 · 1129 bp

Back to Dillenia

Pi 0.0133

MarkerSeek score 55.00

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1129
variable_sites: 11
pi: 0.0133
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.73
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	GGGACTAGAATGAACAGTGCA	GTATGCCATACACACGCCTT	47.6	50.0	57.7	58.3	1004-1284 bp	1.0	71.4
psbM-trnD-GUC_p2	GGGACTAGAATGAACAGTGCA	TGCCATACACACGCCTTATT	47.6	45.0	57.7	57.6	1001-1281 bp	1.0	70.8
psbM-trnD-GUC_p3	GGGACTAGAATGAACAGTGCA	ACACGCCTTATTTACCTGGG	47.6	50.0	57.7	57.6	993-1273 bp	1.0	70.8
psbM-trnD-GUC_p4	GGGACTAGAATGAACAGTGCA	TTCAATTGGTCAGAGCACCG	47.6	50.0	57.7	58.5	966-1246 bp	1.0	70.3
psbM-trnD-GUC_p5	AGGGACTAGAATGAACAGTGC	GTATGCCATACACACGCCTT	47.6	50.0	57.4	58.3	1005-1285 bp	1.0	69.1