Hotspot

trnS-GCU-trnG-GCC

LSC · 8782-9828 · 1047 bp

Back to Dillenia

Pi 0.128

MarkerSeek score 94.00

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 1047
variable_sites: 105
pi: 0.128
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 94.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	CCGACGCTTTAATCCACTCA	ACGAATCGCACTTTTACCACT	50.0	42.9	58.0	58.2	935-1112 bp	1.0	82.1
trnS-GCU-trnG-GCC_p2	CCGACGCTTTAATCCACTCA	ACGAATCGCACTTTTACCAC	50.0	45.0	58.0	56.8	935-1112 bp	1.0	82.0
trnS-GCU-trnG-GCC_p3	ACGGAAAGAGAGGGATTCGA	ACGAATCGCACTTTTACCACT	50.0	42.9	58.2	58.2	993-1170 bp	1.0	80.7
trnS-GCU-trnG-GCC_p4	ACGGAAAGAGAGGGATTCGA	ACGAATCGCACTTTTACCAC	50.0	45.0	58.2	56.8	993-1170 bp	1.0	80.6
trnS-GCU-trnG-GCC_p5	ACGAAAACGGAAAGAGAGGG	ACGAATCGCACTTTTACCACT	50.0	42.9	57.8	58.2	999-1176 bp	1.0	80.5