Hotspot

rps16-trnQ-UUG

LSC · 6443-7420 · 978 bp

Back to Dillenia

Pi 0.0043

MarkerSeek score 65.90

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 978
variable_sites: 4
pi: 0.0043
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	TCCAATGGTGGGCGTAAAAA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.0	58.1	1093-1658 bp	1.0	82.3
rps16-trnQ-UUG_p2	TTCCAATGGTGGGCGTAAAA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.0	58.1	1094-1659 bp	1.0	82.3
rps16-trnQ-UUG_p3	TCCAATGGTGGGCGTAAAAA	AGAAATCTCAATGGGGCGTG	45.0	50.0	58.0	58.2	1152-1717 bp	1.0	80.9
rps16-trnQ-UUG_p4	TTCCAATGGTGGGCGTAAAA	AGAAATCTCAATGGGGCGTG	45.0	50.0	58.0	58.2	1153-1718 bp	1.0	80.9
rps16-trnQ-UUG_p5	ATGGTGGGCGTAAAAATCCA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.8	58.1	1089-1654 bp	1.0	80.6