Hotspot

rpl16

LSC · 78627-80123 · 1497 bp

Back to Diheteropogon

Pi 0.00891

MarkerSeek score 47.00

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1497
variable_sites: 29
pi: 0.00891
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	GATTACCAGCGGCTCCTATG	GGGTCGTCTAGCAGGAAAAG	55.0	55.0	57.9	58.0	80-2008 bp	1.0	83.4
rpl16_p2	TTATACCGTCGTCGCCTTTG	GGGTCGTCTAGCAGGAAAAG	50.0	55.0	58.1	58.0	80-2146 bp	1.0	83.1
rpl16_p3	GATTACCAGCGGCTCCTATG	TCCCATTCTGCCCTGTGATA	55.0	50.0	57.9	58.1	80-1833 bp	1.0	83.0
rpl16_p4	TTTCTAGAGGCATTTGGGGC	GGGTCGTCTAGCAGGAAAAG	50.0	55.0	57.9	58.0	80-2080 bp	1.0	82.8
rpl16_p5	TCAGCAATAGTGTCCTTGCC	GGGTCGTCTAGCAGGAAAAG	50.0	55.0	57.9	58.0	80-2451 bp	1.0	82.5