Hotspot

ndhH

SSC · 115750-116931 · 1182 bp

Back to Diheteropogon

Pi 0.0113

MarkerSeek score 56.30

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 28
pi: 0.0113
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	TGGGTACAATCCATATAAGTCCG	TCGTTTTGAGGAATTCATGGA	43.5	38.1	57.5	55.5	80-1337 bp	1.0	42.1
ndhH_p2	GGGTACAATCCATATAAGTCCGT	TCGTTTTGAGGAATTCATGGA	43.5	38.1	57.5	55.5	80-1336 bp	1.0	42.1
ndhH_p3	TGGGTACAATCCATATAAGTCCG	TCGTTTTGAGGAATTCATGGAA	43.5	36.4	57.5	56.3	80-1337 bp	1.0	42.1
ndhH_p4	GGGTACAATCCATATAAGTCCGT	TCGTTTTGAGGAATTCATGGAA	43.5	36.4	57.5	56.3	80-1336 bp	1.0	42.1
ndhH_p5	ATGGGTACAATCCATATAAGTCCG	TCGTTTTGAGGAATTCATGGA	41.7	38.1	58.0	55.5	80-1338 bp	1.0	42.1