Hotspot

psbE-petL

LSC · 63156-64412 · 1257 bp

Back to Diheteropogon

Pi 0.00546

MarkerSeek score 58.80

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1257
variable_sites: 15
pi: 0.00546
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAAAAGAACGTTCTCCCGTG	AAGCAGCCAGTAGAAAACCG	50.0	50.0	57.3	58.5	80-1331 bp	1.0	76.0
psbE-petL_p2	CAAAAGAACGTTCTCCCGTG	GCAGCCAGTAGAAAACCGAA	50.0	50.0	57.3	58.5	80-1329 bp	1.0	76.0
psbE-petL_p3	CAGCAAAAGAACGTTCTCCC	AAGCAGCCAGTAGAAAACCG	50.0	50.0	57.3	58.5	80-1334 bp	1.0	75.8
psbE-petL_p4	CAGCAAAAGAACGTTCTCCC	GCAGCCAGTAGAAAACCGAA	50.0	50.0	57.3	58.5	80-1332 bp	1.0	75.8
psbE-petL_p5	AGCAAAAGAACGTTCTCCCG	AAGCAGCCAGTAGAAAACCG	50.0	50.0	58.8	58.5	80-1333 bp	1.0	75.1