Hotspot

petA-psbJ

LSC · 61461-62386 · 926 bp

Back to Diheteropogon

Pi 0.0143

MarkerSeek score 65.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 926
variable_sites: 30
pi: 0.0143
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTCGC	ACGGTTCGTATTCTGGATTGG	50.0	47.6	57.9	58.1	80-1064 bp	1.0	76.2
petA-psbJ_p2	GGCCTTTTGTTCTTCTTCGC	GGTTCGTATTCTGGATTGGGT	50.0	47.6	57.9	57.7	80-1062 bp	1.0	74.7
petA-psbJ_p3	GGCCTTTTGTTCTTCTTCGC	CGGTTCGTATTCTGGATTGG	50.0	50.0	57.9	56.3	80-1063 bp	1.0	71.5
petA-psbJ_p4	CGTCCAAGGCCTTTTGTTCT	ACGGTTCGTATTCTGGATTGG	50.0	47.6	58.7	58.1	80-1071 bp	1.0	71.1
petA-psbJ_p5	GGCCTTTTGTTCTTCTTCGC	CGGTTCGTATTCTGGATTGGG	50.0	52.4	57.9	58.8	80-1063 bp	1.0	70.6