Hotspot

rbcL-psaI

LSC · 57241-58194 · 954 bp

Back to Diheteropogon

Pi 0.00996

MarkerSeek score 52.30

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 954
variable_sites: 19
pi: 0.00996
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	TGCAAATGGAGTGCTGAACT	AGGTACGAAAATAGAGGGTAAGT	45.0	39.1	58.0	56.5	80-1083 bp	1.0	49.4
rbcL-psaI_p2	TGCAAATGGAGTGCTGAACT	AAGGTACGAAAATAGAGGGT	45.0	40.0	58.0	53.3	80-1084 bp	1.0	48.4
rbcL-psaI_p3	GCCGCAGCTTGTGAAATATG	AGGTACGAAAATAGAGGGTAAGT	50.0	39.1	58.2	56.5	80-1062 bp	1.0	48.2
rbcL-psaI_p4	TGCAAATGGAGTGCTGAACT	AAAGGTACGAAAATAGAGGGT	45.0	38.1	58.0	54.3	80-1085 bp	1.0	47.7
rbcL-psaI_p5	GCCGCAGCTTGTGAAATATG	AAGGTACGAAAATAGAGGGT	50.0	40.0	58.2	53.3	80-1063 bp	1.0	47.2