Hotspot

rpl16

LSC · 77732-79212 · 1481 bp

Back to Digitaria

Pi 0.0128

MarkerSeek score 52.80

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1481
variable_sites: 28
pi: 0.0128
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	GATTACCAGCGGCTCCTATG	TCCCATTCTGCCCTGTGATA	55.0	50.0	57.9	58.1	1809-1834 bp	1.0	80.5
rpl16_p2	CTCAATTCTTCGGCAATCGC	TCCCATTCTGCCCTGTGATA	50.0	50.0	57.9	58.1	2041-2066 bp	1.0	80.3
rpl16_p3	TCAGCAATAGTGTCCTTGCC	TCCCATTCTGCCCTGTGATA	50.0	50.0	57.9	58.1	2252-2277 bp	1.0	80.1
rpl16_p4	TTTCTAGAGGCATTTGGGGC	TCCCATTCTGCCCTGTGATA	50.0	50.0	57.9	58.1	1881-1906 bp	1.0	79.9
rpl16_p5	GCAATAGTGTCCTTGCCCAT	TCCCATTCTGCCCTGTGATA	50.0	50.0	58.2	58.1	2249-2274 bp	1.0	79.1