Hotspot

psaJ-rps18

LSC · 65020-65775 · 756 bp

Back to Digitaria

Pi 0.00814

MarkerSeek score 62.50

Primer available yes

Label psaJ-rps18

Feature Metadata

label_name: psaJ-rps18
feature_type: igs
region: LSC
length_bp: 756
variable_sites: 9
pi: 0.00814
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rps18_p1	TTTGGGGCTTTAGCAGGTTT	TGGATTTACGAAAGGGTTGCT	45.0	42.9	57.9	57.9	864-886 bp	1.0	73.1
psaJ-rps18_p2	ATGGTTTGGGGCTTTAGCAG	TGGATTTACGAAAGGGTTGCT	50.0	42.9	58.4	57.9	868-890 bp	1.0	70.3
psaJ-rps18_p3	TTTGGGGCTTTAGCAGGTTT	TGGATTTACGAAAGGGTTGC	45.0	45.0	57.9	56.3	864-886 bp	1.0	68.9
psaJ-rps18_p4	GTTTGGGGCTTTAGCAGGTT	TGGATTTACGAAAGGGTTGCT	50.0	42.9	58.7	57.9	865-887 bp	1.0	68.5
psaJ-rps18_p5	TATCTCTCTGTAGCACCCGT	TGGATTTACGAAAGGGTTGCT	50.0	42.9	57.3	57.9	900-922 bp	1.0	67.9