Hotspot

ycf4-cemA

LSC · 58661-59044 · 384 bp

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Pi 0.0106

MarkerSeek score 65.10

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 384
variable_sites: 6
pi: 0.0106
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	ACAAAAAGCTGCCGAATTGG	AGAGAAAGAGACTCCCCAGG	45.0	55.0	57.8	57.8	502-523 bp	1.0	80.1
ycf4-cemA_p2	CGAATTGGCCTCTTTCTTGC	AGAGAAAGAGACTCCCCAGG	50.0	55.0	57.7	57.8	490-511 bp	1.0	79.2
ycf4-cemA_p3	GAATTGGCCTCTTTCTTGCG	AGAGAAAGAGACTCCCCAGG	50.0	55.0	57.7	57.8	489-510 bp	1.0	79.2
ycf4-cemA_p4	CGCGTGCCAATTGAAGTATT	AGAGAAAGAGACTCCCCAGG	45.0	55.0	57.5	57.8	471-492 bp	1.0	77.2
ycf4-cemA_p5	ACAAAAAGCTGCCGAATTGG	CCCAGGGCAAAAGTACGATA	45.0	50.0	57.8	57.3	488-509 bp	1.0	76.5