Hotspot

atpA-trnR-UCU

LSC · 36652-36820 · 169 bp

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Pi 0.028

MarkerSeek score 68.50

Primer available yes

Label atpA-trnR-UCU

Feature Metadata

label_name: atpA-trnR-UCU
feature_type: igs
region: LSC
length_bp: 169
variable_sites: 6
pi: 0.028
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-trnR-UCU_p1	TGAACGGTTTTCCCTTCAGG	GGTTCAAATCCTATTGGACGC	50.0	47.6	58.0	57.3	196-222 bp	1.0	72.6
atpA-trnR-UCU_p2	AGGAACAGCTTGAACGGTTT	GGTTCAAATCCTATTGGACGC	45.0	47.6	57.9	57.3	206-232 bp	1.0	72.3
atpA-trnR-UCU_p3	TAGCAAGACATTCACCGAGC	GGTTCAAATCCTATTGGACGC	50.0	47.6	58.0	57.3	256-282 bp	1.0	72.2
atpA-trnR-UCU_p4	AGCTTGAACGGTTTTCCCTT	GGTTCAAATCCTATTGGACGC	45.0	47.6	57.9	57.3	200-226 bp	1.0	72.2
atpA-trnR-UCU_p5	ATTCACCGAGCAAGCAGAAA	GGTTCAAATCCTATTGGACGC	45.0	47.6	58.1	57.3	247-273 bp	1.0	71.5