Hotspot

trnD-GUC-psbM

LSC · 15799-16860 · 1062 bp

Back to Digitaria

Pi 0.0188
MarkerSeek score 57.40
Primer available yes
Label trnD-GUC-psbM

Feature Metadata

label_name
trnD-GUC-psbM
feature_type
igs
region
LSC
length_bp
1062
variable_sites
24
pi
0.0188
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-psbM_p1 CGCTATCGTCTAGTGATGCC TCCCCCTCCATGGATTCTTT 55.0 50.0 58.0 58.0 1759-2135 bp 1.0 85.2
trnD-GUC-psbM_p2 GGCGCTATCGTCTAGTGATG TCCCCCTCCATGGATTCTTT 55.0 50.0 58.0 58.0 1761-2137 bp 1.0 85.2
trnD-GUC-psbM_p3 CGCTATCGTCTAGTGATGCC TTTCTCCCCCTCCATGGATT 55.0 50.0 58.0 58.0 2118-2139 bp 0.666667 68.9
trnD-GUC-psbM_p4 CGCTATCGTCTAGTGATGCC TTTTCTCCCCCTCCATGGAT 55.0 50.0 58.0 58.0 2119-2140 bp 0.666667 68.9
trnD-GUC-psbM_p5 GGCGCTATCGTCTAGTGATG TTTCTCCCCCTCCATGGATT 55.0 50.0 58.0 58.0 2120-2141 bp 0.666667 68.9