Hotspot

trnG-UCC-trnM-CAU

LSC · 13482-14555 · 1074 bp

Back to Digitaria

Pi 0.0168
MarkerSeek score 65.20
Primer available yes
Label trnG-UCC-trnM-CAU

Feature Metadata

label_name
trnG-UCC-trnM-CAU
feature_type
igs
region
LSC
length_bp
1074
variable_sites
27
pi
0.0168
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnM-CAU_p1 ACGAATCGCACTTTTACCACT CGCTAAGTGAGAAGCCCTTT 42.9 50.0 58.2 57.9 1186-1807 bp 1.0 72.1
trnG-UCC-trnM-CAU_p2 ACGAATCGCACTTTTACCAC CGCTAAGTGAGAAGCCCTTT 45.0 50.0 56.8 57.9 1186-1807 bp 1.0 72.0
trnG-UCC-trnM-CAU_p3 ACGAATCGCACTTTTACCACT CCTTCCGTCTTACCAAAGCA 42.9 50.0 58.2 57.8 1215-1836 bp 1.0 71.4
trnG-UCC-trnM-CAU_p4 ACGAATCGCACTTTTACCACT TGCCTTCCGTCTTACCAAAG 42.9 50.0 58.2 57.8 1217-1838 bp 1.0 71.4
trnG-UCC-trnM-CAU_p5 ACGAATCGCACTTTTACCAC CCTTCCGTCTTACCAAAGCA 45.0 50.0 56.8 57.8 1215-1836 bp 1.0 71.3