Hotspot

ycf1

SSC · 123285-128663 · 5379 bp

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Pi 0.0141

MarkerSeek score 48.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5379
variable_sites: 164
pi: 0.0141
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGTCTCGGCGAAAATCTGAT	AAGGACTCGGCTGTGTAATG	50.0	50.0	58.1	57.6	695 bp	1.0	81.3
ycf1_p2	GGTCTCGGCGAAAATCTGAT	CAAGGACTCGGCTGTGTAAT	50.0	50.0	58.1	57.6	696 bp	1.0	81.3
ycf1_p3	GGTCTCGGCGAAAATCTGAT	AGGACTCGGCTGTGTAATGA	50.0	50.0	58.1	58.4	694 bp	1.0	80.8
ycf1_p4	CGCGCTTTTTCCTTTCTGTT	AAGGACTCGGCTGTGTAATG	45.0	50.0	57.9	57.6	951 bp	0.4	52.4
ycf1_p5	CGCGCTTTTTCCTTTCTGTT	CAAGGACTCGGCTGTGTAAT	45.0	50.0	57.9	57.6	952 bp	0.4	52.4