Hotspot

ndhF

SSC · 109587-111833 · 2247 bp

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Pi 0.0136

MarkerSeek score 52.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2247
variable_sites: 66
pi: 0.0136
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GCAGAATACCGTCACCCATT	GAGGTAAGATCCGGTGAATCG	50.0	52.4	58.0	58.0	2853-2859 bp	1.0	73.9
ndhF_p2	CGAGTTACAGAGACGGGCTA	CCCCCTTCTCTTTCCTTTCG	55.0	55.0	58.6	57.9	2982-2999 bp	0.8	68.3
ndhF_p3	CGAGTTACAGAGACGGGCTA	CAAGCTCCCCCTTCTCTTTC	55.0	55.0	58.6	57.9	2988-2998 bp	0.6	60.0
ndhF_p4	TTTCGAGTTACAGAGACGGG	CCCCCTTCTCTTTCCTTTCG	50.0	55.0	57.0	57.9	2985-2995 bp	0.6	57.1
ndhF_p5	TTTCGAGTTACAGAGACGGG	CAAGCTCCCCCTTCTCTTTC	50.0	55.0	57.0	57.9	2991-2993 bp	0.4	48.8