Hotspot

trnN-GUU-trnR-ACG

IRa · 129069-129670 · 602 bp

Back to Digitalis

Pi 0.0105

MarkerSeek score 66.00

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 602
variable_sites: 12
pi: 0.0105
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	TGGGTTGAGAACGGGAATTG	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.1	57.8	784 bp	1.0	78.7
trnN-GUU-trnR-ACG_p2	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	694 bp	1.0	78.4
trnN-GUU-trnR-ACG_p3	GGGTTGAGAACGGGAATTGA	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	783 bp	1.0	77.9
trnN-GUU-trnR-ACG_p4	CGATTGGTCGTAGGTTCGAA	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.7	57.8	698 bp	1.0	77.5
trnN-GUU-trnR-ACG_p5	TTCCTCAGTAGCTCAGTGGT	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.7	57.8	737 bp	1.0	77.1