Hotspot

ndhD-psaC

SSC · 116544-116636 · 93 bp

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Pi 0.0677

MarkerSeek score 66.30

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 93
variable_sites: 15
pi: 0.0677
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	CCAAGCTGCTAAAGTCGCTA	AGATGTGAATCCGCATGTCC	50.0	50.0	58.0	58.0	524-525 bp	1.0	83.1
ndhD-psaC_p2	AAAAAGTAAAAGGTCGCGCG	AGATGTGAATCCGCATGTCC	45.0	50.0	58.0	58.0	620-621 bp	1.0	82.6
ndhD-psaC_p3	CCAAGCTGCTAAAGTCGCTA	GGTTAGAGCCCTTATGTGCC	50.0	55.0	58.0	58.0	1160-1166 bp	1.0	82.1
ndhD-psaC_p4	AAAAAGTAAAAGGTCGCGCG	GGTTAGAGCCCTTATGTGCC	45.0	55.0	58.0	58.0	1256-1262 bp	1.0	81.9
ndhD-psaC_p5	AACAATCCGATTTGCCCACT	GGTTAGAGCCCTTATGTGCC	45.0	55.0	58.1	58.0	1231-1237 bp	1.0	81.5