Hotspot

ndhF-rpl32

SSC · 111834-112557 · 724 bp

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Pi 0.0198

MarkerSeek score 64.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 724
variable_sites: 31
pi: 0.0198
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACACACTCCAACCAATTCCC	TTTGGGGTGGATTGATGACG	50.0	50.0	58.0	58.2	1787-1890 bp	1.0	85.0
ndhF-rpl32_p2	CACACTCCAACCAATTCCCA	TTTGGGGTGGATTGATGACG	50.0	50.0	58.0	58.2	1786-1889 bp	1.0	85.0
ndhF-rpl32_p3	ACACACTCCAACCAATTCCC	TGAATCGGGTCGGGTAAGAT	50.0	50.0	58.0	58.2	1350-1433 bp	1.0	84.9
ndhF-rpl32_p4	AAGCAGCACACAGTGTAACA	TTTGGGGTGGATTGATGACG	45.0	50.0	58.0	58.2	2010-2113 bp	1.0	84.6
ndhF-rpl32_p5	AGCAGCACACAGTGTAACAA	TTTGGGGTGGATTGATGACG	45.0	50.0	58.0	58.2	2009-2112 bp	1.0	84.6