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petA-psbJ

LSC · 63217-64238 · 1022 bp

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Pi 0.0176

MarkerSeek score 75.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1022
variable_sites: 37
pi: 0.0176
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GAGAAGGTTCAATTGGCCGA	TCCGGATTAGGTTCATCCCT	50.0	50.0	58.2	57.5	1040-1079 bp	1.0	81.4
petA-psbJ_p2	GAGAAGGTTCAATTGGCCGA	CCGGATTAGGTTCATCCCTG	50.0	55.0	58.2	57.4	1039-1078 bp	1.0	80.9
petA-psbJ_p3	AGAAGGTTCAATTGGCCGAA	TCCGGATTAGGTTCATCCCT	45.0	50.0	57.7	57.5	1039-1078 bp	1.0	80.5
petA-psbJ_p4	AGAAGGTTCAATTGGCCGAA	CCGGATTAGGTTCATCCCTG	45.0	55.0	57.7	57.4	1038-1077 bp	1.0	80.1
petA-psbJ_p5	ACGTGTCCAAGGTCTTTTGT	TCCGGATTAGGTTCATCCCT	45.0	50.0	57.6	57.5	1119-1158 bp	1.0	79.0