Hotspot

ndhC-trnV-UAC

LSC · 50358-51485 · 1128 bp

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Pi 0.0244
MarkerSeek score 70.90
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1128
variable_sites
61
pi
0.0244
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGGGCTAAAATCCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1245-1267 bp 1.0 84.5
ndhC-trnV-UAC_p2 TTCTCCGGCCCTTTCCTAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.4 57.8 1266-1288 bp 1.0 81.8
ndhC-trnV-UAC_p3 GGGGCTAAAATCCCGGAAAT GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.9 57.5 1244-1266 bp 1.0 81.8
ndhC-trnV-UAC_p4 GGGGCTAAAATCCCGGAAAT GTTCGAGTCCGTATAGCCCT 50.0 55.0 57.9 58.7 1233-1255 bp 1.0 80.7
ndhC-trnV-UAC_p5 CCCTTTCCTAATCGGGGCTA AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.6 57.8 1258-1280 bp 1.0 80.5