Hotspot

trnL-UAA-trnF-GAA

LSC · 47744-48084 · 341 bp

Back to Digitalis

Pi 0.0205

MarkerSeek score 70.00

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 341
variable_sites: 14
pi: 0.0205
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AATCGTGAGGGTTCAAGTCC	TCTACCAGCTGAGCTATCCC	50.0	55.0	57.5	58.0	389-407 bp	1.0	82.4
trnL-UAA-trnF-GAA_p2	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	425-443 bp	1.0	81.5
trnL-UAA-trnF-GAA_p3	AATCGTGAGGGTTCAAGTCC	TCAGTCCTCTGCTCTACCAG	50.0	55.0	57.5	57.9	401-419 bp	1.0	81.2
trnL-UAA-trnF-GAA_p4	AATCGTGAGGGTTCAAGTCC	CTGTGCCAGGAACCAGATTT	50.0	50.0	57.5	58.1	442-460 bp	1.0	81.0
trnL-UAA-trnF-GAA_p5	AATCGTGAGGGTTCAAGTCC	GTGCCAGGAACCAGATTTGA	50.0	50.0	57.5	58.1	440-458 bp	1.0	81.0