Hotspot

trnT-UGU-trnL-UAA

LSC · 46418-47172 · 755 bp

Back to Digitalis

Pi 0.0125

MarkerSeek score 64.90

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 755
variable_sites: 21
pi: 0.0125
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	813-866 bp	1.0	80.4
trnT-UGU-trnL-UAA_p2	AATAGTCAAAAGCCCGCTGT	CCGTAGCGTCTACCGATTTC	45.0	55.0	57.8	58.2	866-919 bp	1.0	79.7
trnT-UGU-trnL-UAA_p3	ATAGTCAAAAGCCCGCTGTC	CCGTAGCGTCTACCGATTTC	50.0	55.0	58.3	58.2	865-918 bp	1.0	79.2
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	55.0	50.0	57.9	57.6	807-860 bp	1.0	79.1
trnT-UGU-trnL-UAA_p5	AATAGTCAAAAGCCCGCTGT	CGTCTACCGATTTCGCCATA	45.0	50.0	57.8	57.6	860-913 bp	1.0	78.4