Hotspot

psbM-trnD-GUC

LSC · 29570-30105 · 536 bp

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Pi 0.0196

MarkerSeek score 63.50

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 536
variable_sites: 22
pi: 0.0196
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AAATGCAAGAATATTTACTTCCA	TCAATTGGTTAGAGCACCGC	26.1	50.0	52.7	58.6	609-623 bp	1.0	45.6
psbM-trnD-GUC_p2	AATGCAAGAATATTTACTTCCAT	TCAATTGGTTAGAGCACCGC	26.1	50.0	52.4	58.6	608-622 bp	1.0	45.6
psbM-trnD-GUC_p3	AAATGCAAGAATATTTACTTCCAT	TCAATTGGTTAGAGCACCGC	25.0	50.0	53.3	58.6	609-623 bp	1.0	45.6
psbM-trnD-GUC_p4	AAATGCAAGAATATTTACTTCCA	AATTGGTTAGAGCACCGCC	26.1	52.6	52.7	58.1	607-621 bp	1.0	45.6
psbM-trnD-GUC_p5	AATGCAAGAATATTTACTTCCAT	AATTGGTTAGAGCACCGCC	26.1	52.6	52.4	58.1	606-620 bp	1.0	45.6