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psbK-psbI

LSC · 7475-7830 · 356 bp

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Pi 0.0183

MarkerSeek score 66.00

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 356
variable_sites: 14
pi: 0.0183
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CTTTTGTTTGGCAAGCTGCT	ATTCTTCACGTCCCGGATTC	45.0	50.0	58.1	57.7	489-511 bp	1.0	83.3
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	ATTCTTCACGTCCCGGATTC	50.0	50.0	57.9	57.7	484-506 bp	1.0	83.3
psbK-psbI_p3	TGGCAAGCTGCTGTAAGTTT	ATTCTTCACGTCCCGGATTC	45.0	50.0	58.3	57.7	481-503 bp	1.0	81.3
psbK-psbI_p4	TTGGCAAGCTGCTGTAAGTT	ATTCTTCACGTCCCGGATTC	45.0	50.0	58.3	57.7	482-504 bp	1.0	81.3
psbK-psbI_p5	TTTGGCAAGCTGCTGTAAGT	ATTCTTCACGTCCCGGATTC	45.0	50.0	58.3	57.7	483-505 bp	1.0	81.3