Hotspot

rps16-trnQ-UUG

LSC · 6088-6886 · 799 bp

Back to Digitalis

Pi 0.0148

MarkerSeek score 63.40

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 799
variable_sites: 27
pi: 0.0148
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	GTTCTTCAAGTCGCACGTTG	GAAATTGAAATGGGGCGTGG	50.0	50.0	58.0	58.0	937-969 bp	1.0	84.0
rps16-trnQ-UUG_p2	GTTCTTCAAGTCGCACGTTG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	879-911 bp	1.0	83.7
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	GAAATTGAAATGGGGCGTGG	45.0	50.0	58.1	58.0	930-962 bp	1.0	82.9
rps16-trnQ-UUG_p4	ATCGTGTTCTTCAAGTCGCA	GAAATTGAAATGGGGCGTGG	45.0	50.0	57.9	58.0	942-974 bp	1.0	82.8
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	872-904 bp	1.0	82.6