Hotspot

trnK-UUU-rps16

LSC · 4220-4955 · 736 bp

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Pi 0.0152
MarkerSeek score 68.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
736
variable_sites
24
pi
0.0152
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT TTTCCTTGAAAAAGGGGCCC 50.0 50.0 58.2 58.6 845-865 bp 1.0 80.2
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT TTTCCTTGAAAAAGGGGCCC 50.0 50.0 58.2 58.6 844-864 bp 1.0 80.2
trnK-UUU-rps16_p3 TCTAGCCGCACTTAAAAGCC TTTCCTTGAAAAAGGGGCCC 50.0 50.0 58.3 58.6 859-879 bp 1.0 79.3
trnK-UUU-rps16_p4 TCAGTCGTGGTCTTCCAAAC TTTCCTTGAAAAAGGGGCCC 50.0 50.0 57.8 58.6 930-950 bp 1.0 79.1
trnK-UUU-rps16_p5 CAGTCGTGGTCTTCCAAACT TTTCCTTGAAAAAGGGGCCC 50.0 50.0 57.8 58.6 929-949 bp 1.0 79.0