Pi
0.000659
MarkerSeek score
54.40
Primer available
yes
Label
ycf1
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| ycf1_p1 | ACAGGAGGTTTTTCATGACT | TGTAGACATGGGATTTTGTTCT | 40.0 | 36.4 | 54.5 | 55.4 | 2479-2509 bp | 1.0 | 41.1 |
| ycf1_p2 | AACAGGAGGTTTTTCATGACT | TGTAGACATGGGATTTTGTTCT | 38.1 | 36.4 | 55.3 | 55.4 | 2480-2510 bp | 1.0 | 41.1 |
| ycf1_p3 | ACAGGAGGTTTTTCATGACTT | TGTAGACATGGGATTTTGTTCT | 38.1 | 36.4 | 55.3 | 55.4 | 2479-2509 bp | 1.0 | 41.1 |
| ycf1_p4 | ACAGGAGGTTTTTCATGACT | GGGATTTTGTTCTATGATTTGTGC | 40.0 | 37.5 | 54.5 | 57.1 | 2470-2500 bp | 1.0 | 41.1 |
| ycf1_p5 | ACAGGAGGTTTTTCATGACT | TTGTAGACATGGGATTTTGTTCT | 40.0 | 34.8 | 54.5 | 56.1 | 2480-2510 bp | 1.0 | 41.1 |
| ycf1_p1 | CTGAATATTCCGCGACGTGA | GTGTGATGAGAAAACTGAAAG | 50.0 | 38.1 | 58.2 | 53.1 | 243-279 bp | 1.0 | 44.3 |
| ycf1_p2 | TCTGAATATTCCGCGACGTG | GTGTGATGAGAAAACTGAAAG | 50.0 | 38.1 | 58.2 | 53.1 | 244-280 bp | 1.0 | 44.3 |
| ycf1_p3 | AGTTGAAGCCGTGTTTCTGA | GTGTGATGAGAAAACTGAAAG | 45.0 | 38.1 | 57.7 | 53.1 | 259-295 bp | 1.0 | 44.3 |
| ycf1_p4 | TGAATATTCCGCGACGTGAC | GTGTGATGAGAAAACTGAAAG | 50.0 | 38.1 | 58.4 | 53.1 | 242-278 bp | 1.0 | 44.3 |
| ycf1_p5 | TTCTGAATATTCCGCGACGT | GTGTGATGAGAAAACTGAAAG | 45.0 | 38.1 | 57.4 | 53.1 | 245-281 bp | 1.0 | 44.3 |