Hotspot

rpoC2

LSC · 17828-21970 · 4143 bp

Back to Diervilla

Pi 0.000241

MarkerSeek score 54.00

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4143
variable_sites: 1
pi: 0.000241
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	CTTGATCCTTGTGGCTCGAA	AGTGATTGCTGAGATTCGCG	50.0	50.0	57.9	58.7	233 bp	1.0	73.8
rpoC2_p2	TGATCCTTGTGGCTCGAAAG	AGTGATTGCTGAGATTCGCG	50.0	50.0	57.9	58.7	231 bp	1.0	73.8
rpoC2_p3	CTAGACTGGATCTGCATGGC	AGTGATTGCTGAGATTCGCG	55.0	50.0	57.8	58.7	206 bp	1.0	73.3
rpoC2_p4	TAGACTGGATCTGCATGGCT	AGTGATTGCTGAGATTCGCG	50.0	50.0	58.2	58.7	205 bp	1.0	73.1
rpoC2_p5	GGCTCGAAAGGGAGACTAGA	AGTGATTGCTGAGATTCGCG	55.0	50.0	58.2	58.7	221 bp	1.0	72.8