Hotspot

rpl2

IRa · 158181-159358 · 1178 bp

Back to Diervilla

Pi NA

MarkerSeek score 46.20

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: IRa
length_bp: 1178
variable_sites: 0
pi: NA
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.00
markerseek_score: 46.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	CGCTTCAACCGGGTTATTCT	TTGTCCACCCTTTCCAAGTG	50.0	50.0	58.3	57.9	1186 bp	0.5	57.3
rpl2_p2	CGCTTCAACCGGGTTATTCT	CTTCCCAAACCGTACAAGCT	50.0	50.0	58.3	58.1	1088 bp	0.5	57.0
rpl2_p3	ATCATTACGCTTCAACCGGG	TTGTCCACCCTTTCCAAGTG	50.0	50.0	58.3	57.9	1193 bp	0.5	56.8
rpl2_p4	ATCATTACGCTTCAACCGGG	CTTCCCAAACCGTACAAGCT	50.0	50.0	58.3	58.1	1095 bp	0.5	56.4
rpl2_p5	CGCTTCAACCGGGTTATTCT	TCAAAACCCCTTCCCAAACC	50.0	50.0	58.3	58.2	1097 bp	0.5	56.2