Hotspot

ndhC

LSC · 52392-52754 · 363 bp

Back to Diervilla

Pi 0

MarkerSeek score 22.80

Primer available yes

Label ndhC

Feature Metadata

label_name: ndhC
feature_type: gene
region: LSC
length_bp: 363
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 22.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC_p1	GGTTGTTCGATCAAGTAAGGGA	CCACGTTCATAGGAGTGTGT	45.5	50.0	58.1	57.3	484 bp	1.0	57.5
ndhC_p2	TGGGTTGTTCGATCAAGTAAGG	CCACGTTCATAGGAGTGTGT	45.5	50.0	58.3	57.3	486 bp	1.0	55.4
ndhC_p3	GGTTGTTCGATCAAGTAAGGGA	TCCACGTTCATAGGAGTGTG	45.5	50.0	58.1	57.0	485 bp	1.0	55.2
ndhC_p4	GGGTTGTTCGATCAAGTAAGG	CCACGTTCATAGGAGTGTGT	47.6	50.0	56.6	57.3	485 bp	1.0	55.0
ndhC_p5	GGTTGTTCGATCAAGTAAGGG	CCACGTTCATAGGAGTGTGT	47.6	50.0	56.6	57.3	484 bp	1.0	55.0