Hotspot

psaI-ycf4

LSC · 62353-62777 · 425 bp

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Pi 0

MarkerSeek score 55.30

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 425
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	ATTTCCGGCAATTGCAATGG	GGATAACGGCCCAGCATAAA	45.0	50.0	58.0	58.0	567-568 bp	1.0	79.7
psaI-ycf4_p2	ATTTCCGGCAATTGCAATGG	TTGTTTTTCGAGACCCCGTT	45.0	45.0	58.0	58.0	543-544 bp	1.0	79.6
psaI-ycf4_p3	GGCAATTGCAATGGCTTCTT	GGATAACGGCCCAGCATAAA	45.0	50.0	57.9	58.0	561-562 bp	1.0	79.1
psaI-ycf4_p4	GGCAATTGCAATGGCTTCTT	TTGTTTTTCGAGACCCCGTT	45.0	45.0	57.9	58.0	537-538 bp	1.0	79.0
psaI-ycf4_p5	GGCCTAGTATTTCCGGCAAT	GGATAACGGCCCAGCATAAA	50.0	50.0	57.7	58.0	575-576 bp	1.0	77.8