Hotspot

rpoB-trnC-GCA

LSC · 28220-29501 · 1282 bp

Back to Diervilla

Pi 0.00078

MarkerSeek score 59.20

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1282
variable_sites: 1
pi: 0.00078
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TCCCTCATTTCCATCCTGGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.7	58.1	1362 bp	1.0	77.1
rpoB-trnC-GCA_p2	GTTCTTCCATCAAGCCCTGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.5	58.1	1441 bp	1.0	75.4
rpoB-trnC-GCA_p3	AGTTCTTCCATCAAGCCCTG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.5	58.1	1442 bp	1.0	75.4
rpoB-trnC-GCA_p4	TCCCTCATTTCCATCCTGGA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.7	58.4	1344 bp	1.0	74.9
rpoB-trnC-GCA_p5	CTTCCATCAAGCCCTGATCA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.3	58.1	1438 bp	1.0	73.6