Hotspot

trnH-GUG-psbA

LSC · 83-441 · 359 bp

Back to Diervilla

Pi 0
MarkerSeek score 54.00
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
359
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG CTCTAGACCTAGCCGCTCTT 55.0 55.0 58.1 58.0 424-425 bp 1.0 79.0
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA CTCTAGACCTAGCCGCTCTT 50.0 55.0 57.9 58.0 438-439 bp 1.0 78.9
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC CTCTAGACCTAGCCGCTCTT 50.0 55.0 57.9 58.0 437-438 bp 1.0 78.9
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC CTCTAGACCTAGCCGCTCTT 50.0 55.0 57.9 58.0 439-440 bp 1.0 78.9
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG TAGACCTAGCCGCTCTTGAA 55.0 50.0 58.1 57.9 421-422 bp 1.0 78.0