Hotspot

rpl32-trnL-UAG

SSC · 113135-114036 · 902 bp

Back to Didymocarpus

Pi 0.0257

MarkerSeek score 73.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 902
variable_sites: 72
pi: 0.0257
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TTTTTGTGCGACAAACAAAT	TTTAGGAGATGACATGCCGC	30.0	50.0	53.4	58.0	765-1025 bp	1.0	52.5
rpl32-trnL-UAG_p2	TTTTTGTGCGACAAACAAAT	TCCTAAGAGCAGCGTGTCTA	30.0	50.0	53.4	57.9	710-970 bp	1.0	52.2
rpl32-trnL-UAG_p3	TTTTTGTGCGACAAACAAAT	AGCAGCGTGTCTACCAATTT	30.0	45.0	53.4	57.8	703-963 bp	1.0	51.7
rpl32-trnL-UAG_p4	TTTTTGTGCGACAAACAAAT	CCTAAGAGCAGCGTGTCTAC	30.0	55.0	53.4	57.8	709-969 bp	1.0	51.6
rpl32-trnL-UAG_p5	TTTTTGTGCGACAAACAAAT	CTAAGAGCAGCGTGTCTACC	30.0	55.0	53.4	57.8	708-968 bp	1.0	51.6