Hotspot

atpB-rbcL

LSC · 54932-55702 · 771 bp

Back to Didymocarpus

Pi 0.0112

MarkerSeek score 67.40

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 771
variable_sites: 35
pi: 0.0112
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GGAAACCCCAGAACCAGAAG	AACACCCGCTTTGAATCCAA	55.0	45.0	58.1	58.3	848-886 bp	1.0	83.7
atpB-rbcL_p2	ATGATTTGGACGATACGCCC	AACACCCGCTTTGAATCCAA	50.0	45.0	58.1	58.3	889-927 bp	1.0	83.3
atpB-rbcL_p3	CCGGACCTATGATTTGGACG	AACACCCGCTTTGAATCCAA	55.0	45.0	58.1	58.3	897-935 bp	1.0	83.2
atpB-rbcL_p4	GGCGGAAAGGCTACATCTAG	AACACCCGCTTTGAATCCAA	55.0	45.0	57.8	58.3	919-957 bp	1.0	82.8
atpB-rbcL_p5	GGAAACCCCAGAACCAGAAG	TAACACCCGCTTTGAATCCA	55.0	45.0	58.1	57.4	849-887 bp	1.0	81.6