Hotspot

ndhC-trnV-UAC

LSC · 50902-51944 · 1043 bp

Back to Didymocarpus

Pi 0.0198

MarkerSeek score 70.90

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1043
variable_sites: 72
pi: 0.0198
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CCCCCGAAATGAAAAATGCC	GCACGTTGGGTCTTTGAAAC	50.0	50.0	58.0	58.2	1047-1235 bp	1.0	87.7
ndhC-trnV-UAC_p2	CTCTGGCCCCTTTCTAATCG	GCACGTTGGGTCTTTGAAAC	55.0	50.0	57.8	58.2	1076-1264 bp	1.0	85.7
ndhC-trnV-UAC_p3	CCCCCGAAATGAAAAATGCC	TGCACGTTGGGTCTTTGAAA	50.0	45.0	58.0	58.5	1048-1236 bp	1.0	84.7
ndhC-trnV-UAC_p4	GGCTAAAACCCCCGAAATGA	GCACGTTGGGTCTTTGAAAC	50.0	50.0	58.2	58.2	1055-1243 bp	0.909091	82.6
ndhC-trnV-UAC_p5	GGCTAAAACCCCCGAAATGA	TGCACGTTGGGTCTTTGAAA	50.0	45.0	58.2	58.5	1056-1244 bp	0.909091	79.6