Hotspot

rps4-trnT-UGU

LSC · 46510-46900 · 391 bp

Back to Didymocarpus

Pi 0.0236

MarkerSeek score 78.20

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 391
variable_sites: 33
pi: 0.0236
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	GTTAGTCCTGGTAAAGCCCC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	57.6	57.9	492-536 bp	1.0	85.0
rps4-trnT-UGU_p2	GTTAGTCCTGGTAAAGCCCC	ATGGTCATCGGTTCGATTCC	55.0	50.0	57.6	57.8	460-504 bp	1.0	84.5
rps4-trnT-UGU_p3	TTAGTCCTGGTAAAGCCCCC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.7	57.9	491-535 bp	1.0	82.7
rps4-trnT-UGU_p4	GATCACTTCCGGCTTTAGGC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.7	57.9	521-565 bp	1.0	82.5
rps4-trnT-UGU_p5	GTTAGTCCTGGTAAAGCCCC	CCGCTTAGCTCAGAGGTTAG	55.0	55.0	57.6	57.5	500-544 bp	1.0	82.0