Hotspot

trnG-UCC-trnR-UCU

LSC · 10262-10443 · 182 bp

Back to Didymocarpus

Pi 0.0451

MarkerSeek score 72.70

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 182
variable_sites: 30
pi: 0.0451
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	TGTCCTATCCATTAGACAATG	50.0	38.1	58.0	52.4	252-279 bp	1.0	52.0
trnG-UCC-trnR-UCU_p2	ACCCTTAGCCTTCCAAGCTA	TGTCCTATCCATTAGACAATG	50.0	38.1	58.0	52.4	258-285 bp	1.0	52.0
trnG-UCC-trnR-UCU_p3	CTAACGATGCGGGTTCGATT	TGTCCTATCCATTAGACAATG	50.0	38.1	58.4	52.4	241-268 bp	1.0	52.0
trnG-UCC-trnR-UCU_p4	TAACGATGCGGGTTCGATTC	TGTCCTATCCATTAGACAATG	50.0	38.1	58.4	52.4	240-267 bp	1.0	52.0
trnG-UCC-trnR-UCU_p5	TAACCCTTAGCCTTCCAAGC	TGTCCTATCCATTAGACAATG	50.0	38.1	57.2	52.4	260-287 bp	1.0	52.0