Hotspot

psbA-trnK-UUU

LSC · 1486-1711 · 226 bp

Back to Didymocarpus

Pi 0.0238

MarkerSeek score 65.90

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 226
variable_sites: 21
pi: 0.0238
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	CCCCATAGGCTTTCGCTTTC	CTAGTTCCGGGTTCGAATCC	55.0	55.0	59.0	57.8	285-308 bp	1.0	80.0
psbA-trnK-UUU_p2	TCGCGTCTCTCTAAAATTGCA	CTAGTTCCGGGTTCGAATCC	42.9	55.0	58.0	57.8	267-290 bp	1.0	79.8
psbA-trnK-UUU_p3	CCCCATAGGCTTTCGCTTT	CTAGTTCCGGGTTCGAATCC	52.6	55.0	57.8	57.8	285-308 bp	1.0	78.2
psbA-trnK-UUU_p4	CGCGTCTCTCTAAAATTGCAG	CTAGTTCCGGGTTCGAATCC	47.6	55.0	57.7	57.8	266-289 bp	1.0	77.8
psbA-trnK-UUU_p5	CCCATAGGCTTTCGCTTTCG	CTAGTTCCGGGTTCGAATCC	55.0	55.0	59.3	57.8	284-307 bp	1.0	77.1