Hotspot

trnH-GUG-psbA

LSC · 125-426 · 302 bp

Back to Didymocarpus

Pi 0.0495

MarkerSeek score 78.30

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 302
variable_sites: 52
pi: 0.0495
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TCAGTGCTATGCATGGTTCC	55.0	50.0	58.1	58.0	748-799 bp	1.0	87.8
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	TCAGTGCTATGCATGGTTCC	50.0	50.0	57.9	58.0	762-813 bp	1.0	87.6
trnH-GUG-psbA_p3	TATTTGTAGGGCGAACGACG	TCAGTGCTATGCATGGTTCC	50.0	50.0	58.1	58.0	813 bp	0.090909	42.6
trnH-GUG-psbA_p4	TATTTGTAGGGCGAACGACG	CAGTGCTATGCATGGTTCCT	50.0	50.0	58.1	58.0	812 bp	0.090909	42.5
trnH-GUG-psbA_p5	TATTTGTAGGGCGAACGACG	GGTGTAGCTGGTGTATTCGG	50.0	55.0	58.1	58.1	844 bp	0.090909	42.4